Identifying Functional Bioactives

Identifying Functional Bioactives

Research Lead : A/Prof Morten Andersen, Macquarie University

The diversity of chemical structures of bioactive compounds within complex biological mixtures challenges our ability to identify, isolate, formulate and register new active ingredients based on bioactives. Liquid chromatography coupled to mass spectrometry (LC-MS) and related techniques have become state-of-art for chemical analysis in the pharmaceutical and biotechnology sectors but high costs and the need for highly qualified analysts has prevented the bioactives industry from routinely accessing this capability. In addition, whilst extraction methods for many principal ingredients have been established widely, extracted molecules are not always efficacious. Analytical methods that probe both molecular and macromolecular structures of bioactives and their interdependence in situ need to be developed and coupled to relevant bioassays to allow rapid lead candidate selection.

FAAB Industry Centre Higher Degree Research projects will focus on developing and implementing advanced analytical methods for discovering, identifying and structurally characterising macromolecular bioactives systems from industry partners. The projects will implement small-molecule methods, proteomics, glycomics, scattering, spectroscopy, and microscopy to identify active molecular forms of putative bioactives and to determine how to process raw material (often a by-product) into the required form to be effective. High-throughput screening protocols will be developed for discovery of new bioactives and sophisticated analysis will support the design of novel products and new quality control instrumentation, methods and technology. The holy grail will be developing separation-free molecular characterisation methods that can enable the utilisation of simpler bioassays.

Enhancing Bioactives

FAAB increases understanding of bioactives in their complex product matrix, at a molecular and microbiome level, to enhance potential therapeutic effectiveness, and together with commercial partners, derive new potential from food waste streams and controlled processing.

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Delivering Bioactives

FAAB directs our specialised analytical and experimental frameworks towards effective delivery of bioactives, combatting poor solubility, enhancing bioavailability, and investigating diverse matrices, formulation and administration routes.

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